Live Imaging

live

Hepatic Stellate Cell Live Imaging
We also have state of the art facility to extend liver drug discovery research to the next levels of activities.  State of the art live imaging platform coupled with on stage CO2 incubator and hypoxia chamber is available to track live cells.
1. Confocal Microscopy: High end optical images with a complete analysis of activated HSC cells under the treatment of liver drugs is performed using immunostaining technique.

2. Flow cytometry and cell sorting platform: The effect of the drugs on cellular targets is carried out using flow cytometry and FACS.

REFERENCES

1. Yin C, Evason KJ, Asahina K, Stainier DY.Hepatic stellate cells in liver development, regeneration, and cancer. J Clin Invest. 2013 May;123(5):1902-10.
2. Mederacke I, Hsu CC, Troeger JS, Huebener P, Mu X, Dapito DH, Pradere JP, Schwabe RF.Fate tracing reveals hepatic stellate cells as dominant contributors to liver fibrosis independent of its aetiology. Nat Commun. 2013;4:2823.