Genomics

genomics

Hepatic Stellate Cell Genomics
1. Sanger sequencing: DNA sequencing in activated HSCs under the treatment of drug is performed using Sangers dideoxy method.
2. Next Generation Sequencing: High throughput sequencing using an illumina miseq machine is carried out with complete analysis and interpretation in hepatic stellate cells under the treatment of drug.
3. Analysis of big data: Big data analytics is the steps of analysing large data sets containing a variety of data types to unravel hidden patterns and unknown correlations. The findings and its interpretations may lead to new horizons of drug discovery; developing a a more effective drug for liver diseses.

REFERENCES

1. Okuyama H, Shimahara Y, Kawada N.The hepatic stellate cell in the post-genomic era.Histol Histopathol. 2002 Apr;17(2):487-95.
2. El Taghdouini A, Sørensen AL, Reiner AH, Coll M, Verhulst S, Mannaerts I, Øie CI, Smedsrød B, Najimi M, Sokal E, Luttun A, Sancho-Bru P, Collas P, van Grunsven LA.Genome-wide analysis of DNA methylation and gene expression patterns in purified, uncultured human liver cells and activated hepatic stellate cells.Oncotarget. 2015 Sep 29;6(29):26729-45.